Authors
Yoshitaka
Kosaka
,
Kyoto Prefectural Institute of Agricultural Biotechnology, Kyoto 619-0244
;
Bo-Song
Ryang
,
Laboratory of Plant Pathology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka 599-8531
;
Takashi
Kobori
,
Kyoto Prefectural Institute of Agricultural Biotechnology, Kyoto 619-0244
;
Hiroshi
Shiomi
,
Takii Plant Breeding and Experimental Station, Shiga 520-3231
;
Hisao
Yasuhara
,
Kyoto Biken Laboratories, Inc., Kyoto 611-0041
; and
Mitsunobu
Kataoka
,
Kyoto Prefectural Institute of Agricultural Biotechnology, Kyoto 619-0244, Japan
ABSTRACT
To cross-protect cucumber plants from Zucchini yellow mosaic virus (ZYMV), we used cold treatment to obtain an attenuated isolate of ZYMV, designated ZYMV-2002. ZYMV-2002 was obtained from a virulent ZYMV isolate after repeated low temperature treatment at 12.5 to 15°C followed by five cycles of single-plant transfer. The isolate produced very mild or no symptoms on cucurbit plants. In addition, inoculated cucumber plants had very similar fruit productivity to healthy control plants under field conditions. During field experiments in 2002 and 2003, when other viruses were also present, protected plants significantly suppressed infection with ZYMV, progression of disease severity, and reduction of fruit yield and quality. These results demonstrate that ZYMV-2002 is a potentially useful attenuated ZYMV isolate for reducing the impact of ZYMV.
