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Biological and Molecular Variability Among High Plains virus Isolates

August 2004 , Volume 88 , Number  8
Pages  824 - 829

Dallas L. Seifers , Professor, Kansas State University, Agricultural Research Center-Hays 67601-9228 ; Y.-M. She , Department of Physics, University of Manitoba, Winnipeg, Manitoba, Canada ; Tom L. Harvey , Professor, Department of Entomology, Kansas State University, Manhattan 66506 ; T. J. Martin , Professor, Kansas State University, Agricultural Research Center-Hays ; S. Haber , Cereal Research Centre, Agriculture & Agri-Food Canada, Winnipeg, Manitoba, Canada ; W. Ens and K. G. Standing , Department of Physics, University of Manitoba, Winnipeg ; and Raymond Louie , United States Department of Agriculture-Agricultural Research Service and D. T. Gordon , Professor, Emeritus, Department of Plant Pathology, The Ohio State University, Wooster 44691

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Accepted for publication 30 March 2004.

The High Plains virus (HPV), vectored by the wheat curl mite (WCM) (Aceria tosichella), causes a severe disease of maize (Zea mays) in the U. S. High Plains. In the present study, five HPV isolates from five states were separated from co-infecting Wheat streak mosaic virus and their molecular and biological variability studied. Molecular studies involved time-of-flight mass spectrometry (TOFMS) to determine amino acid sequence variability of the 32-kDa nucleoprotein (32 np) of the isolates. Biological studies involved testing the ability of the five HPV isolates to infect a maize line previously shown to have resistance. Inoculations of the HPV isolates were conducted using vascular puncture inoculation (VPI) and viruliferous WCM. TOFMS analyses demonstrated an 18-amino acid sequence in the isolates at the N-terminus of the 32 np, the presence of amino acid sequence differences among the isolates, and variability among amino acid sequences of the 32 np of some isolates. Three of the five HPV isolates infected the resistant maize inbred, B73, using VPI, and two of the same three HPV isolates infected this line using WCM inoculation, albeit low numbers of plants were infected by each technique.

© 2004 The American Phytopathological Society