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Resistance in Mango Against Infection by Ceratocystis fimbriata

August 2014 , Volume 104 , Number  8
Pages  820 - 833

Leonardo Araujo, Wilka Messner Silva Bispo, Isaías Severino Cacique, Wiler Ribas Moreira, and Fabrício Ávila Rodrigues

Viçosa Federal University, Department of Plant Pathology, Laboratory of Host-Pathogen Interaction, Viçosa, Minas Gerais State, 36570-900, Brazil.

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Accepted for publication 10 February 2014.

This study was designed to characterize and describe host cell responses of stem tissue to mango wilt disease caused by the fungus Ceratocystis fimbriata in Brazil. Disease progress was followed, through time, in inoculated stems for two cultivars, ‘Ubá’ (field resistant) and ‘Haden’ (field susceptible). Stem sections from inoculated areas were examined using fluorescence light microscopy and transmission and scanning electron microscopy, coupled with energy-dispersive X-ray microanalysis. Tissues from Ubá colonized by C. fimbriata had stronger autofluorescence than those from Haden. The X-ray microanalysis revealed that the tissues of Ubá had higher levels of insoluble sulfur and calcium than those of Haden. Scanning electron microscopy revealed that fungal hyphae, chlamydospores (aleurioconidia), and perithecia-like structures of C. fimbriata were more abundant in Haden relative to Ubá. At the ultrastructural level, pathogen hyphae had grown into the degraded walls of parenchyma, fiber cells, and xylem vessels in the tissue of Haden. However, in Ubá, plant cell walls were rarely degraded and hyphae were often surrounded by dense, amorphous granular materials and hyphae appeared to have died. Taken together, the results of this study characterize the susceptible and resistant basal cell responses of mango stem tissue to infection by C. fimbriata.

Additional keywords: barrier zones, phenolic-like compounds, vascular pathogen.

© 2014 The American Phytopathological Society