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Acquisition and Transmission of Peanut clump virus by Polymyxa graminis on Cereal Species

October 2011 , Volume 101 , Number  10
Pages  1,149 - 1,158

Benjamin Dieryck, Jeannine Weyns, Diane Doucet, Claude Bragard, and Anne Legrève

Université catholique de Louvain, Earth and Life Institute, Applied Microbiology–Phytopathology, Croix du Sud 2/3, B-1348 Louvain-la-Neuve, Belgium.

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Accepted for publication 1 May 2011.

The objective of this study was to investigate the specificity of the interactions between Polymyxa graminis, Peanut clump virus (PCV), and cereals, particularly the acquisition and the transmission of the virus by three P. graminis formae speciales. A new strategy has been developed: it involves using sugarcane as the common host for both the virus and its vector in order to produce the viruliferous zoospores of P. graminis f. sp. subtropicalis, temperata, and tropicalis that were then inoculated on cereal species. This experiment enabled the role of P. graminis f. sp. tropicalis and subtropicalis zoospores in PCV transmission to be demonstrated. The efficiency of this transmission was shown to vary, depending on the P. graminis special forms. Interestingly, the high transmission of the PCV isolate from Burkina Faso by an isolate of P. graminis f. sp. tropicalis from Niger on pearl millet suggests that there is a coevolution mechanism in this pathosystem. The study also provides evidence that the host plant species in which Polymyxa zoospores are produced could affect the infectivity of the vector. Finally, using Polymyxa quantitation by quantitative reverse-transcription polymerase chain reaction and in situ observations of the virus, the study demonstrates the independence of the development of PCV and its vector in the host plants.

© 2011 The American Phytopathological Society