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In Vitro-Transcribed Chrysanthemum stunt viroid RNA Is Infectious to Chrysanthemum and Other Plants

January 2009 , Volume 99 , Number  1
Pages  58 - 66

Yosuke Matsushita and Kumar K. R. Penmetcha

First author: Research Team for Growth and Flowering, National Institute of Floricultural Science, 2-1, Fujimoto, Tsukuba 305-8519, Japan; and second author: Functional Nucleic Acids, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan.

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Accepted for publication 23 September 2008.

Chrysanthemum stunt viroid (CSVd), a noncoding RNA, is known to cause chrysanthemum stunt disease, which affects the yield of flowers. To gain insights into CSVd replication, infection, and the reasons for the spreading of CSVd disease in chrysanthemum plants, we prepared linear CSVd RNA and analyzed its ability to cause disease in chrysanthemum plants. We found that linear CSVd replicated as efficiently as CSVd RNA isolated from the infected chrysanthemum plants. Additionally, the linear CSVd RNA was evaluated for its ability to infect other plants as well, which revealed that CSVd has a wide host range for its replication. Importantly, the CSVd isolated from these hosts is infectious to chrysanthemum plants, and thus potentially contributes to the spreading of the disease to chrysanthemum plants.

© 2009 The American Phytopathological Society