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Engineered Mild Strains of Papaya ringspot virus for Broader Cross Protection in Cucurbits

May 2005 , Volume 95 , Number  5
Pages  533 - 540

Bang-Jau You , Chu-Hui Chiang , Li-Fang Chen , Wei-Chih Su , and Shyi-Dong Yeh

First, third, fourth, and fifth authors: Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan; and second author: Department of Molecular Biotechnology, Da Yeh University, Changhua, Taiwan

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Accepted for publication 16 December 2004.

Papaya ringspot virus (PRSV) HA5-1, a mild mutant of type P Hawaii severe strain (PRSV P-HA), has been widely used for the control of PRSV type P strains in papaya, but did not provide practical protection against PRSV type W strains in cucurbits. In order to widen the protection effectiveness against W strains, chimeric mild strains were constructed from HA5-1 to carry the heterologous 3′ genomic region of a type W strain W-CI. Virus accumulation of recombinants and their crossprotection effectiveness against W-CI and P-HA were investigated. In horn melon and squash plants, the recombinant carrying both the heterologous coat protein (CP) coding region and the 3′ untranslated region (3′UTR), but not the heterologous CP coding region alone, significantly enhanced the protection against W-CI. The heterologous 3′UTR alone is critical for the enhancement of the protection against W-CI in horn melon, but not in zucchini squash. In papaya, the heterologous CP coding region or 3′UTR alone, but not both together, significantly reduced the effectiveness of cross protection against P-HA. Our recombinants provide broader protection against both type W and P strains in cucurbits; however, the protective effectiveness is also affected by virus accumulation, the organization of the 3′ genomic region, and host factors.

© 2005 The American Phytopathological Society