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Genetic Mapping of Pathogenicity and Aggressiveness of Gibberella zeae (Fusarium graminearum) Toward Wheat

May 2004 , Volume 94 , Number  5
Pages  520 - 526

Christian Joseph R. Cumagun , Robert L. Bowden , James E. Jurgenson , John F. Leslie , and Thomas Miedaner

First and fifth authors: State Plant Breeding Institute (720), University of Hohenheim, D-70593 Stuttgart, Germany; second author: USDAARS, Plant Science and Entomology Research Unit, 4008 Throckmorton Hall, Manhattan, KS 66506-5502; third author: Department of Biology, University of Northern Iowa, Cedar Falls 50614; and fourth author: Department of Plant Pathology, Kansas State University, Manhattan 66506-5502

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Accepted for publication 27 January 2004.

Gibberella zeae is the major fungal pathogen of Fusarium head blight of wheat and produces several mycotoxins that are harmful to humans and domesticated animals. We identified loci associated with pathogenicity and aggressiveness on an amplified fragment length polymorphism based genetic map of G. zeae in a cross between a lineage 6 nivalenol producer from Japan and a lineage 7 deoxynivalenol producer from Kansas. Ninety-nine progeny and the parents were tested in the greenhouse for 2 years. Progeny segregated qualitatively (61:38) for pathogenicity:nonpathogenicity, respectively. The trait maps to linkage group IV, which is adjacent to loci that affect colony pigmentation, perithecium production, and trichothecene toxin amount. Among the 61 pathogenic progeny, the amount of disease induced (aggressiveness) varied quantitatively. Two reproducible quantitative trait loci (QTL) for aggressiveness were detected on linkage group I using simple interval analysis. A QTL linked to the TRI5 locus (trichodiene synthase in the trichothecene pathway gene cluster) explained 51% of the variation observed, and a second QTL that was 50 centimorgans away explained 29% of the phenotypic variation. TRI5 is tightly linked to the locus controlling trichothecene toxin type. The two QTLs, however, were likely part of the same QTL using composite interval analysis. Progeny that produced deoxynivalenol were, on average, approximately twice as aggressive as those that produced nivalenol. No transgressive segregation for aggressiveness was detected. The rather simple inheritance of both traits in this interlineage cross suggests that relatively few loci for pathogenicity or aggressiveness differ between lineage 6 and 7.

Additional keywords: AFLP, cereal, deoxynivalenol, nivalenol, scab, trichothecenes, Triticum aestivum.

The American Phytopathological Society, 2004