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Development of Co-Dominant Amplified Polymorphic Sequence Markers in Rice that Flank the Magnaporthe grisea Resistance Gene Pi7(t) in Recombinant Inbred Line 29

March 2004 , Volume 94 , Number  3
Pages  302 - 307

M. A. Campbell , D. Chen , and P. C. Ronald

First and third authors: Department of Plant Pathology, University of California at Davis, Davis 95616; and second author: DNA LandMarks, Saint-Jean-sur-Richelieu, Quebec, J3B 6Z1, Canada

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Accepted for publication 4 November 2003.

Pi7(t), a dominant blast resistance gene derived from the rice cultivar Moroberekan, confers complete resistance against the fungal pathogen Magnaporthe grisea. Pi7(t) previously was positioned on chromosome 11 by restriction fragment length polymorphism (RFLP) mapping of a recombinant inbred line population. One derivative of this population, recombinant inbred line (RIL)29, was designated as the representative line for Pi7(t). A segregating F2 population was created from RIL29 in order to determine the location of Pi7(t). The new mapping data indicate a position for Pi7(t) 30 centimorgans distal to the original location. Pi7(t) shares a common position with the previously mapped Pi1 M. grisea resistance gene. RIL29 carries DNA not derived from either parent used to create the RIL population at the newly assigned Pi7(t) locus. RFLP analysis has identified a possible donor source.

© 2004 The American Phytopathological Society