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A Laboratory Simulation for Vectoring of Trichosporon pullulans by Conidia of Botrytis cinerea

December 2002 , Volume 92 , Number  12
Pages  1,293 - 1,299

Darryl W. M. Cook

Sustainable Disease Management Group, The Horticultural and Food Research Institute of New Zealand, Ruakura Research Center, Private Bag 3123, Hamilton, New Zealand

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Accepted for publication 24 July 2002.

A mechanism that could contribute to the suppression of Botrytis cinerea during pathogen sporulation was examined in this study. Yeasts capable of binding to B. cinerea were formulated with a cellulose carrier and applied to sporulating colonies of the pathogen. The particles from this yeast/cellulose product attached to B. cinerea conidia in the sporulating colony. Inoculum from treated colonies was harvested and applied to tomato stem tissue to test for subsequent pathogenicity. Disease development from inoculum obtained from cultures that had been treated with Trichosporon pullulans was significantly retarded (P = 0.0001) compared with cellulose-only controls. However, between 5 and 11% of conidia applied were attached to yeast cells. The removal of conidia not attached to yeast resulted in inoculum composed of >90% of conidia attached to yeast, and from this inoculum, disease development was significantly retarded (P < 0.05). When inoculum from treated B. cinerea colonies was applied to nutrient limiting agar and then incubated, the B. cinerea conidia germinated, and yeast cells infested the new hyphal growth. Constraints of the formulation of the yeast used in this study, and the implications of this vectoring approach for the suppression of B. cinerea during pathogen sporulation are discussed.

Additional keywords: adhesion, settling chamber, sporulation, timing of inoculum application, yeast.

© 2002 The American Phytopathological Society