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Genetic Analysis of Sensitivity to a Pyrenophora tritici-repentis Necrosis-Inducing Toxin in Durum and Common Wheat

April 1999 , Volume 89 , Number  4
Pages  293 - 297

J. A. Anderson , R. J. Effertz , J. D. Faris , L. J. Francl , S. W. Meinhardt , and B. S. Gill

First author: Department of Agronomy and Plant Genetics, 411 Borlaug Hall, University of Minnesota, St. Paul 55108; second and fourth authors: Department of Plant Pathology, North Dakota State University, Fargo 58105; third and sixth authors: Department of Plant Pathology, Throckmorton Plant Science Center, Kansas State University, Manhattan 66506-5502; and fifth author: Biochemistry Department, North Dakota State University, Fargo 58105

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Accepted for publication 4 January 1999.

The fungus Pyrenophora tritici-repentis produces a toxin (Ptr ToxA) that causes rapid cell necrosis in sensitive wheat genotypes. A single recessive gene (tsn1) on chromosome 5BL in common wheat confers insensitivity to this toxin. Our objectives were to analyze the allelic relationships of genotypes that have shown insensitivity to a P. tritici-repentis necrosis-inducing toxin, map the gene for insensitivity to the necrosis-inducing factor produced by P. tritici-repentis in a durum wheat population, and determine the reaction to P. tritici-repentis of aneuploid genotypes that do not contain the gene. Greenhouse-grown plants of seven populations from crosses of insensitive genotypes; an F2 population of durum wheat; and ‘Chinese Spring’ aneuploid, substitution, and deletion lines were infiltrated with Ptr ToxA. All crosses involving insensitive genotypes failed to produce sensitive progeny, indicating that the same gene is present in these genotypes. The gene for insensitivity in the durum population was mapped to the same region on 5BL as in common wheat using restriction fragment length polymorphism markers. ‘Chinese Spring’, its homoeologous group 5 nullisomic-tetrasomic stocks, and 5BL deletion lines were insensitive to the toxin. Substitution of a 5B chromosome from sensitive genotypes into ‘Chinese Spring’ resulted in sensitivity. Therefore, insensitivity is not conferred by a gene product per se, but rather conferred by absence of a gene for sensitivity.

Additional keywords: host-pathogen interactions, molecular markers, resistance, yellow leaf spot.

The American Phytopathological Society, 1999