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Quantification of Within-Field Spread of Soybean Mosaic Virus in Soybean Using Strain-Specific Monoclonal Antibodies

September 1998 , Volume 88 , Number  9
Pages  895 - 901

Forrest W. Nutter , Jr. , Patricia M. Schultz , and John H. Hill

Department of Plant Pathology, Iowa State University, Ames 50011

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Accepted for publication 10 June 1998.

Strain-specific monoclonal antibodies were used to follow the temporal increase and spatial spread of soybean mosaic virus (SMV) strain G-5 released from a point source. The use of strain-specific monoclonal antibodies allowed discrimination of within-field temporal and spatial spread of SMV strain G-5 from non-G-5 SMV isolates that originated from exogenous field sources. SMV isolates originating from exogenous sources have potential to alter the temporal and spatial pattern of within-field virus spread, which could potentially affect the choice of models used to quantify within-field pathogen spread. Analysis of SMV epidemics in field-plot experiments indicated that the logistic model was the most appropriate model to describe and compare the temporal spread of SMV among years. On the basis of ordinary runs analyses, within-field spread of SMV strain G-5 was random in 1991 and 1994, but was mostly aggregated in 1992 and 1993. Non-G-5 SMV isolates arising from exogenous sources displayed a random spatial pattern over time. This is the first study in which pathogen incidence (detection of SMV using strain-specific monoclonal antibodies) as opposed to disease incidence (based on symptoms) was employed to monitor and model SMV spread in time and space.

Additional keywords: potyvirus , risk assessment , statistical modeling , virus epidemiology .

© 1998 The American Phytopathological Society