Link to home

Genetic Analysis of Metalaxyl Insensitivity Loci in Phytophthora infestans Using Linked DNA Markers

October 1997 , Volume 87 , Number  10
Pages  1,034 - 1,040

Anna-Liisa Fabritius , Richard C. Shattock , and Howard S. Judelson

First and third authors: Department of Plant Pathology, University of California, Riverside 92521; and second author: School of Biological Sciences, University of Wales, Bangor, Gwynedd LL572UW, United Kingdom

Go to article:
Accepted for publication 8 July 1997.

Previous studies indicated that incompletely dominant loci determine insensitivity by oomycetes to phenylamide fungicides such as metalaxyl. To compare the bases of insensitivity in different strains of the late blight pathogen, Phytophthora infestans, crosses were performed between sensitive isolates and isolates from Mexico, the Netherlands, and the United Kingdom that displayed varying levels of insensitivity. Segregation analyses indicated that metalaxyl insensitivity was determined primarily by one locus in each isolate, and that two of the isolates were heterozygous and the other homozygous for the insensitive allele. Metalaxyl insensitivity was also affected by the segregation of additional loci of minor effect. DNA markers linked to insensitivity were obtained by bulked segregant analysis using random amplified polymorphic DNA (RAPD) markers and the Dutch and Mexican crosses. By studying the linkage relationships between these markers and the insensitivity in each cross by RAPD or restriction fragment length polymorphism analysis, it appeared that the same chromosomal locus conferred insensitivity in the Mexican and Dutch isolates. However, a gene at a different chromosomal position was responsible for insensitivity in the British isolate.

Additional keywords: fungicide resistance, quantitative trait.

© 1997 The American Phytopathological Society