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Lettuce mosaic virus Pathogenicity Determinants in Susceptible and Tolerant Lettuce Cultivars Map to Different Regions of the Viral Genome

June 2001 , Volume 14 , Number  6
Pages  804 - 810

Elise Redondo , 1 Renate Krause-Sakate , 1 Shu-Jun Yang , 1 Hervé Lot , 2 Olivier Le Gall , 1 and Thierry Candresse 1

1Virologie, UMR GDPP, IBVM, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France; 2Pathologie Végétale, INRA, Domaine St. Maurice, BP 94, 84143 Montfavet Cedex, France

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Accepted 8 February 2001.

Full-length infectious cDNA clones were constructed for two isolates (LMV-0 and LMV-E) of Lettuce mosaic virus (LMV), a member of the genus Potyvirus. These two isolates differ in pathogenicity in susceptible and tolerant-resistant lettuce cultivars. In susceptible plants, LMV-0 induces mild mosaic symptoms, whereas LMV-E induces severe stunting, leaf deformation, and a necrotic mosaic. In plants carrying either of the two probably allelic recessive resistance genes mo11 or mo12, LMV-0 is restricted partially to the inoculated leaves. When a systemic invasion does occur, however, symptoms fail to develop. LMV-E overcomes the protection afforded by the resistance genes, resulting in systemic mosaic symptoms. Analysis of the behavior of recombinants constructed between the two virus isolates determined that the HC-Pro protein of LMV-E causes the severe stunting and necrotic mosaic induced by this isolate in susceptible cultivars. In contrast, the ability to overcome mo1 resistance and induce symptoms in the resistant-tolerant cultivars was mapped to the 3′ half of the LMV-E genome. These results indicate that the ability to induce severe symptoms and to overcome the protection afforded by the recessive genes mo11 or mo12 are independent phenomena.

Additional keywords: 35S, leaf necrosis, resistance breaking, VPg.

© 2001 The American Phytopathological Society