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Mutants of Tobacco mosaic virus with Temperature-Sensitive Coat Proteins Induce Heat Shock Response in Tobacco Leaves

July 2001 , Volume 14 , Number  7
Pages  914 - 917

Harald Jockusch , Christiane Wiegand , Birgit Mersch , and Daniela Rajes

Developmental Biology and Molecular Pathology, University of Bielefeld, D-33501 Bielefeld, Germany

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Accepted 19 March 2001.

We analyzed, with respect to heat shock proteins (HSPs), systemically reacting tobacco leaves inoculated with Tobacco mosaic virus (TMV), wild-type vulgare, and temperature-sensitive coat protein (CP) mutants Ni 118 (P20L) and flavum (D19A), kept at 23 or 30°C. HSP18 and HSP70 mRNAs and proteins were induced with temperature-sensitive CP mutants after 1 to 2 days at 30°C. After 4 to 6 days, HSP70 was also induced at 23°C. The induction of HSPs paralleled the amount of insoluble TMV CP in leaf extracts, indicating that denatured TMV CP by itself induces a heat-shock response.

© 2001 The American Phytopathological Society