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Cloning and Characterization of a Bean Udp-Glucosyltransferase cDNA Expressed During Plant-Bacterial Interactions

January 2001 , Volume 14 , Number  1
Pages  90 - 92

T. A. Sullivan , J. L. Jakobek , and P. B. Lindgren

Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, U.S.A.

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Accepted 13 September 2000.

A cDNA clone, which corresponds to an RNA transcript that accumulates in bean during the hypersensitive reaction, was isolated and designated Hra25 (for hypersensitive reaction associated). Hra25 is predicted to encode a UDP-glucosyltransferase. Northern analysis was used to study Hra25 transcript accumulation in bean in response to incompatible and compatible strains of Pseudomonas syringae, an Hrp¯ mutant, and wounding. Our data suggest that the Hra25 transcript is activated in response to specific avr-derived signals as well as non-avr, general signals.

© 2001 The American Phytopathological Society