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Phytophthora Resistance Through Production of a Fungal Protein Elicitor (β-Cryptogein) in Tobacco

January 1998 , Volume 11 , Number  1
Pages  64 - 67

David Tepfer , 1 Catherine Boutteaux , 1 Catherine Vigon , 1 Sylvie Aymes , 2 Valérie Perez , 2 Michael J. O'Donohue , 2 Jean-Claude Huet , 2 and Jean-Claude Pernollet 2

1Biologie de la Rhizosphère, INRA, Route de St Cyr, F-78026 Versailles Cedex, France; 2Biochimie et Structure des Protéines, INRA, Domaine de Vilvert, F-78352 Jouy-en-Josas Cedex, France

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Accepted 26 September 1997.

Transformation of tobacco with a gene encoding the fungal elicitor protein, β-cryptogein, resulted in resistance to the pathogen Phytophthora parasitica var. nicotianae. Resistance was improved when the foreign gene was in the hemizygous state, and a single amino acid substitution that reduced the necrotic effects of the protein also conferred some resistance.

© 1998 The American Phytopathological Society