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Salicylate-Independent Lesion Formation in Arabidopsis lsd Mutants

July 1997 , Volume 10 , Number  5
Pages  531 - 536

Michelle D. Hunt , 1 Terrence P. Delaney , 1 Robert A. Dietrich , 2 Kris B. Weymann , 1 Jeffery L. Dangl , 2 and John A. Ryals 1

1Biotechnology and Genomics Center, Novartis Crop Protection, Inc., P.O. Box 12257, Research Triangle Park, NC 27709 U.S.A.; 2University of North Carolina, Department of Biology, 108 Coker Hall, Chapel Hill 27514 U.S.A.

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Accepted 25 February 1997.

In many interactions of plants with pathogens, the primary host defense reaction is accompanied by plant cell death at the site of infection. The resulting lesions are correlated with the establishment of an inducible resistance in plants called systemic acquired resistance (SAR), for which salicylic acid (SA) accumulation is a critical signaling event in Arabidopsis and tobacco. In Arabidopsis, the lesions simulating disease (lsd) mutants spontaneously develop lesions in the absence of pathogen infection. Furthermore, lsd mutants express SAR marker genes when lesions are present and are resistant to the same spectrum of pathogens as plants activated for SAR by necrogenic pathogen infection. To assess the epistatic relationship between SA accumulation and cell death, transgenic Arabidopsis unable to accumulate SA due to the expression of the salicylate hydroxylase (nahG) gene were used in crosses with the dominant mutants lsd2 or lsd4. Progeny from the crosses were inhibited for SAR gene expression and disease resistance. However, these progeny retained the spontaneous cell death phenotype similar to siblings not expressing nahG. Because lesions form in the absence of SA accumulation for lsd2 and lsd4, a model is suggested in which lesion formation in these two mutants is determined prior to SA accumulation in SAR signal transduction. By contrast, the loss of SAR gene expression and disease resistance in nahG-expressing lsd mutants indicates that these traits are dependent upon SA accumulation in the SAR signal transduction pathway.

Additional keywords: autofluorescence, callose, feedback amplification loop, Peronospora parasitica isolate Noco, PR gene expression.

© 1997 The American Phytopathological Society