Protein Signatures from Wild Type and Reduced Genomic Compliments of Rhizoctonia solani Isolates.
B. Narayanaswamy (1), J. BIRCH (1), S. Bharathan (1), M. Chey (1), R. Connacher (1), B. Michalides (1), A. Long (1), M. Cubeta (2). (1) Indiana Univ of PA, Indiana, PA, U.S.A.; (2) North Carolina State Univ, Raleigh, NC, U.S.A.
Amongst genetically isolated anastomosis groups (AG’s), <i>Rhizoctonia solani, </i>a soil-borne, plant pathogenic fungus, shows an increased genetic variability between these groups. Sub-groups of these AG’s groups can be further differentiated via colony morphology, virulence, genetic sequences, biochemical characteristics and host ranges. Data from specific protocols and standard operating procedures developed for protein purifications of filamentous fungi using ToPI-DIGE kit will be presented. Proteins were purified from select <i>R. solani </i>isolates that included wild-type, heterokaryons, and compared to those that had reduced genomic complements, homokaryons. The concentration and quality of the protein were assayed using Beckam-Coulter DU 800 Spectrophotometer. All such high-quality proteins were subjected to Agilent Protein Chip Technology protein separation and 2D-protein profiling. Preliminary results suggests considerable variation in the protein from the homokaryon and the wild-type isolates. Proteins Spot statistics data from 2D-gel preparations indicated nearly 81% of the proteins were similar while there was an under-expression of 10.4% in the wild-type and 8.7% over-expression in the homokaryon. Additional comparisons are being made between viral-infected versus non-infected <i>R. solani</i> isolates.
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