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Wide hybridisations for blackleg (Leptosphaeria maculans) resistance transfer into oilseed rape (Brassica napus).
H. WINTER (1), M. Mosch (1), F. Marthe (2), H. Peterka (2), O. Schrader (2), H. Budahn (2). (1) Technische Universitaet Dresden, Department of Biology – Molecular Biotechnology, Dresden, Germany; (2) Institute for Breeding Research on Horticultural and Fruit Crops of Julius Kuehn Institute, Federal Research Centre for Cultivated Plants, Quedlinb

Blackleg caused by the ascomycete <i>Leptosphaeria maculans</i> (<i>Phoma lingam</i>) is the most significant disease affecting oilseed rape (<i>Brassica napus</i>) worldwide. Considering climate change, it is expected to become even more relevant in future. To widen the narrow base of oilseed rape resistance, offspring derived from somatic hybrids <i>B. oleracea</i> (+) <i>B. nigra</i> and <i>B. oleracea</i> (+) <i>B. carinata</i>, respectively, are currently characterised and developed towards the <i>B. napus</i> karyotype (genome AACC, 2n=38). The focus of this study is on blackleg resistance behaviour of selected selfing and backcross offspring produced using embryo rescue techniques. Adult plant resistant individuals of different generations, along with susceptible genotypes, were examined cytologically, e. g. by genomic <i>in situ</i> hybridisation (GISH). Furthermore, the most promising genotypes were self pollinated again and backcrossed with <i>B. napus</i> to obtain resistant plants with an AACC background. Moreover, a complete set of nine disomic <i>B. napus</i>-<i>Raphanus sativus</i> addition lines (2n=38<sub>AACC</sub>+2<sub>(R)</sub>), originally developed for nematode resistance transfer, has been examined in blackleg resistance tests. One of these lines showed adult plant resistance similar to <i>R. sativus</i>. GISH results are compared with those obtained earlier from blackleg resistant addition and putative recombination lines derived from interspecific, sexual hybrids between <i>B. napus</i> and <i>Sinapis arvensis</i>, <i>Coincya monensis</i> and <i>B. juncea</i>, respectively. <p><p>Keywords: Fungus, Vegetables, Brassicas (Crucifers)

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