Link to home

Molecular Epidemiology of Plum pox virus in Japan

May 2011 , Volume 101 , Number  5
Pages  567 - 574

Kensaku Maejima, Misako Himeno, Ken Komatsu, Yusuke Takinami, Masayoshi Hashimoto, Shuichiro Takahashi, Yasuyuki Yamaji, Kenro Oshima, and Shigetou Namba

First to ninth authors: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 113-8657, Japan.

Go to article:
Accepted for publication 17 December 2010.

For a molecular epidemiological study based on complete genome sequences, 37 Plum pox virus (PPV) isolates were collected from the Kanto region in Japan. Pair-wise analyses revealed that all 37 Japanese isolates belong to the PPV-D strain, with low genetic diversity (less than 0.8%). In phylogenetic analysis of the PPV-D strain based on complete nucleotide sequences, the relationships of the PPV-D strain were reconstructed with high resolution: at the global level, the American, Canadian, and Japanese isolates formed their own distinct monophyletic clusters, suggesting that the routes of viral entry into these countries were independent; at the local level, the actual transmission histories of PPV were precisely reconstructed with high bootstrap support. This is the first description of the molecular epidemiology of PPV based on complete genome sequences.

© 2011 The American Phytopathological Society