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Xylella fastidiosa Requires Polygalacturonase for Colonization and Pathogenicity in Vitis vinifera Grapevines

April 2007 , Volume 20 , Number  4
Pages  411 - 419

M. Caroline Roper , 1 L. Carl Greve , 2 Jeremy G. Warren , 1 John M. Labavitch , 2 and Bruce C. Kirkpatrick 1

1Department of Plant Pathology and 2Department of Plant Sciences, University of California, Davis. Davis, CA, 95616, U.S.A.

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Accepted 14 November 2006.

Xylella fastidiosa is the causal agent of Pierce's disease of grape, an economically significant disease for the grape industry. X. fastidiosa systemically colonizes the xylem elements of grapevines and is able to breach the pit pore membranes separating xylem vessels by unknown mechanisms. We hypothesized that X. fastidiosa utilizes cell wall degrading enzymes to break down pit membranes, based on the presence of genes involved in plant cell wall degradation in the X. fastidiosa genome. These genes include several β-1,4 endoglucanases, several xylanases, several xylosidases, and one polygalacturonase (PG). In this study, we demonstrated that the pglA gene encodes a functional PG. A mutant in pglA lost pathogenicity and was compromised in its ability to systemically colonize Vitis vinifera grapevines. The results indicate that PG is required for X. fastidiosa to successfully infect grapevines and is a critical virulence factor for X. fastidiosa pathogenesis in grapevine.

Additional keyword: pectinase.

© 2007 The American Phytopathological Society