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The Rhizobium etli amtB Gene Coding for an NH4+ Transporter Is Down-Regulated Early During Bacteroid Differentiation

March 1998 , Volume 11 , Number  3
Pages  188 - 198

Rosarita Taté , 1 Anna Riccio , 1 Mike Merrick , 2 and Eduardo J. Patriarca 1

1International Institute of Genetics and Biophysics, CNR, via Marconi 10, 80125 Naples, Italy; 2Nitrogen Fixation Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom

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Accepted 21 November 1997.

During development of root nodules, Rhizobium bacteria differentiate inside the invaded plant cells into N2-fixing bacteroids. Terminally differentiated bacteroids are unable to grow using the ammonia (NH3 ) produced therein by the nitrogenase complex. Therefore, the nitrogen assimilation activities of bacteroids, including the ammonium (NH4 +) uptake activity, are expected to be repressed during symbiosis. By sequence homology the R. etli amtB (ammonium transport) gene was cloned and sequenced. As previously shown for its counterpart in other organisms, the R. etli amtB gene product mediates the transport of NH4 +. The amtB gene is cotranscribed with the glnK gene (coding for a PII-like protein) from a nitrogen-regulated σ54-dependent promoter, which requires the transcriptional activator NtrC. Expression of the glnKamtB operon was found to be activated under nitrogen-limiting, free-living conditions, but down-regulated just when bacteria are released from the infection threads and before transcription of the nitrogenase genes. Our data suggest that the uncoupling between N2-fixation and NH3 assimilation observed in symbiosomes is generated by a transcriptional regulatory mechanism(s) beginning with the inactivation of NtrC in younger bacteroids.

Additional keywords: Phaseolus vulgaris.

© 1998 The American Phytopathological Society