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The Coat Protein Is Required for the Elicitation of the Capsicum L2 Gene-Mediated Resistance Against the Tobamoviruses

January 1997 , Volume 10 , Number  1
Pages  107 - 113

A. de la Cruz , L. López , F. Tenllado , J. R. Díaz-Ruíz , A. I. Sanz , C. Vaquero , M. T. Serra , and I. García-Luque

Departamento de Biología de Plantas, Centro de Investigaciones Biológicas (CSIC), Velázquez 144, 28006-Madrid, Spain

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Accepted 8 November 1996.

In Capsicum, the resistance against tobamoviruses conferred by the L2 gene is effective against all but one of the known tobamoviruses. Pepper mild mottle virus (PMMoV) is the only virus which escapes its action. To identify the viral factors affecting induction of the hypersensitive reaction (HR) mediated by the Capsicum spp. L2 resistance gene, we have constructed chimeric viral genomes between paprika mild mottle virus (PaMMV) (a virus able to induce the HR) and PMMoV. A hybrid virus with the PaMMV coat protein gene substituted in the PMMoV-S sequences was able to elicit the HR in Capsicum frutescens (L2L2) plants. These data indicate that the sequences that affect induction of the HR mediated by the L2 resistance gene reside in the coat protein gene. Furthermore, a mutant that codes for a truncated coat protein was able to systemically spread in these plants. Thus, the elicitation of the host response requires the coat protein and not the RNA.

Additional keywords: tobamovirus L2 resistance gene.

The American Phytopathological Society, 1997