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Diversified transcriptional modulation of alternative splicing repertoire during rice-Magnaporthe oryzae interactions

Jongbum Jeon: Seoul National University

<div>In post-genome era, one gene to one protein paradigm has shifted to one gene to multiple proteins or functions in whole genome. Among transcript modulating mechanisms, alternative splicing (AS) have potential roles in modulating genomic systems. Although 80.4% of the genome contains introns, only less than 8% of genome was revealed as AS containing genes in <em>Magnaporthe oryzae</em>. Similar to transcriptome alterations, AS genes would be reprogrammed during rice-<em>M. oryzae</em> interaction<em>.</em> To unveil ASome networks, we compared genome-wide transcriptional profiles of AS isoforms of fungal pathogen and host plant. We collected infected rice sheaths under microscope to enrich fungal samples as a sequential manner. These samples included vegetative condition and five different infection stages covering pre-penetration, biotrophic, and nectrotrophic stages. In this study, at least one AS isoform of 4,765 genes were found from total conditions, including 3,042 genes from <em>in planta</em> conditions. Two-thirds of isoform patterns showed intron retention, and an increase of this pattern led to the upsizing of ASome infection stages. Among total stages, the largest amount of stage-specific AS isoforms were found in biotrophic stage<em>. </em>We also identified AS isoforms assigned differential function compared with stages by <em>ab initio</em> proteome. This profiling of ASome provides expanded AS repertoire of <em>M. oryzae</em> and shows genome-wide transcriptomic complexity during rice-<em>M. oryzae</em> interactions.</div>