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Culturable microbiota and metagenome data show distinct microbial assemblage between bacterial wilt disease-suppressive and conducive soils

Ronnie Gicana: National Chung Hsing University

<div>Suppressive soil is one the most interesting examples of community interaction that depict the role of the microbiome in influencing plant fitness towards specific diseases and offer microbiome-mediated protection of crops against soil-borne pathogens. Using high throughput amplicon sequencing of the <em>16S rRNA</em> gene, we compared the culturable microbioata and the metagenome of the bacterial wilt disease-suppressive and -conducive soils identified from several eggplant farms in southern Taiwan. The culturable microbiota showed the abundance of the phylum <em>Firmicutes</em> for both soil phenotypes, and the predominance of the bacterium <em>Bacillus fumarioli</em> in the suppressive soil. The same result was obtained from the metagenomics analysis, which is contrary to the published results indicating the predominance of the phylum <em>Proteobacteria</em> in the soil. Regardless of the source of soil samples, the bacterial wilt disease-suppressive soil was dominated by <em>B. fumarioli</em>. Analysis of the community structure of the two soil phenotypes showed a higher alpha diversity in the conducive compared to the suppressive soil. Non-metric Multidimensional Scaling (NMDS), Principal Coordinates Analysis (PCoA) and Hierarchical Cluster analysis showed significant clustering of the bacterial wilt disease-suppressive from the –conducive soil, indicating distinct microbial assemblage of these two soil phenotypes.</div>

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