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Molecular mechanism of high-temperature resistance to yellow rust in Xiaoyan6

Jiahui Wang: Northwest A&F University

<div></p> <p>Stripe rust, caused by <em>Puccinia striiformis</em> f. sp. <em>tritici</em> (Pst), is a devastating disease of wheat (<em>Triticum aestivum</em>) worldwide. A loss of resistance to stripe rust in the wheat cultivars appeared frequently due to production of new virulent race of Pst constantly, whereas, wheat high-temperature seedling plant (HTSP) resistance to Pst is non-race-specific and durable. We identified 28 high-temperature resistance wheat cultivars to Pst including 13 high-temperature seedling variety from 400 landraces and 92 improved wheat in Northwest China since 1989. Among them, Xiaoyan6 maintains its resistance against stripe rust for nearly 30 years, is a typical durable resistant wheat variety. Xiaoyan6 was selected as a model wheat cultivar to study the HTSP resistance to Pst. The transcriptome of Xiaoyan6 under different temperature treatments were sequenced with Illumina technology, and the molecular mechanism was primarily illuminated by analyzing of different expression genes, KEGG pathway enrichment, and protein interaction. The results indicated that receptor like kinase with the help of heat shock proteins can combined with various effectors by interacting with different types of resistance genes, inducing the HR response of Xiaoyn6. In addition, Pst and temperature were detected by receptor like kinase following by the phosphorylation of kinase, transferring the signal to the WRKY transposon factors through the MAPK cascade, and causing the defense responses. The genes such as <em>TaWRKY70</em>, <em>TaRPM1</em>,<em> TaRPS2</em> were employed to confirm the mechanism. These results can help people understand the mechanism of HTSP, and will make contributions to improve and utilize the resistance of HTSP.</div>