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Towards deciphering host resistance to phytonematodes: transcriptome analysis of a coffee incompatible response to Meloidogyne incognita

Erika Valeria Saliba Albuquerque: Embrapa Recursos Geneticos e Biotecnologia

<div>The root-knot nematode (RKN) <em>Meloidogyne</em> <em>incognita</em> is considered one of the most economically damaging pathogen in coffee crops. Breeding introgression strategies with R genes result in resistance breaking, while chemicals nematicides are considered harmful. We had previously observed that the development of <em>M. incognita</em> in <em>Coffea arabica</em> is prevented by the necrosis of the host cells at 6 days after infection (dpi) in (R) roots, while the feeding sites are being established by the formation of giant cells in (S). Samples from these contrasting materials were sequenced by RNAseq Illumina HiSeq 4000, generating over 800 million 2x100 nt length reads. Differential expression analysis and GO enrichment results point out that some gene families are strongly deregulated in the resistance response. These genes could be important players in the coffee-RKN incompatible interaction and may be potentially used in biotechnological approaches to nematode control.</div>

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