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The composition and expression of Carbohydrate-Active Enzymes in Rhizoctonia cerealis transcriptome

Wei Li: Jiangsu Academy of Agricultural Sciences

<div>The soilborne fungus <em>Rhizoctonia cerealis</em>, which belongs to the binucleate <em>Rhizoctonia</em> anastomosis group AG-DI, is the pathogen of wheat sharp eyespot in the temperate regions of the world with significant impacts on grain yield and quality. Until now, several genome sequences of <em>R. solani </em>isolates which belong to the multinucleate <em>Rhizoctonia</em>, have been published. However, it has very rarely been reported in binucleate <em>Rhizoctonia</em> spp. isolates and has never been described in <em>R. cerealis</em>. In this study, we sequenced the transcriptome and secretome of<em> R. cerealis</em> strain R0301 using Illumina Genome Analyzer sequencing technology, and analyzed the composition and expression of Carbohydrate-Active Enzymes in this fungus. The results indicated that among 21,021 unigene entries of <em>R. cerealis </em>transcriptome, 465 (2.2 %) could match with one CAZyme family. These putative CAZymes include 44 families of carbohydrate esterases (CE), 255 families of glycoside hydrolases (GH), 146 families of glycosyltransferases (GT), and 19 families of polysaccharide lyases (PL). The species and quantities of CAZymes in <em>R. cerealis</em> were similar with the CAZymes in <em>R. solani</em> AG1-IA and AG8 genome, which all of them are the monocotyledon pathogen. We further compared the CAZyme expression patterns in <em>R. cerealis</em> under three different conditions, the results show that many CAZyme genes were significantly up-regulated during the infection of wheat, which indicated that some of the CAZymes would play important roles in the infection process. This study will serve as a resource for future studies to understand the infection mechanism of <em>R. cerealis</em> to wheat.</div>