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Characterization of two new Pseudomonas species isolated from cranberry galls in Massachusetts

Tawny Castaneda: Midwestern University

<div>The American cranberry (<em>Vaccinium macrocarpon</em> Ait.) is an important commercial crop in the US. However, little is known about its ecology, especially with regard to its microbiome or opportunistic pathogens. Cranberry stem galls have previously been associated with harvest damage, and the presence of several phytohormone-producing genera of bacteria, including <em>Pantoea</em>, <em>Enterobacter</em>, and <em>Pseudomonas</em>. Bacteria were isolated from cranberry upright (stem) galls in a Massachusetts bog. Of the isolates collected from galls, we placed two within the genus <em>Pseudomonas</em> by means of comparing 16S rRNA gene sequences, fatty acid methyl ester analysis, metabolic activity, and other phenotypic characteristics. Based on whole genome sequencing, one isolate appears to cluster within the <em>Pseudomonas syringae</em> <em>sensu lato </em>group. The other may represent the first isolate from a previously unknown clade within the genus<em>.</em></div>