The structure and function of the global citrus root-associated microbiome
Jin Xu: University of Florida
<div><strong>The structure and function of the global citrus root-associated microbiome</strong></p> <p>Jin Xu<sup>1,2</sup> Yunzeng Zhang<sup>1</sup>, and Nian Wang<sup>1, *</sup></p> <p><sup>1</sup>Citrus Research and Education Center, Department of Microbiology and Cell Science, IFAS, University of Florida, Lake Alfred, FL</p> <p><sup>2</sup>Citrus Research and Education Center, Department of Plant Pathology, IFAS, University of Florida, Lake Alfred, FL</p> <p><sup>*</sup>Corresponding author: Nian Wang, 700 experiment station road, Lake Alfred, FL 33850</p> <p>Tel: 01-8639568828, fax: 01-8639564631, email: <a href="mailto:nianwang@ufl.edu";>nianwang@ufl.edu<;/a></p> <p><strong>Abstract: </strong>Plant root-associated microbiome plays important roles in biogeochemical cycles and promoting plant growth and health. However, the genomic, taxonomic and functional contents of plant microbiome remain largely unknown. Here we established a large-scale gene catalog of the root-associated microbiome of citrus, a perennial plant, via deep metagenome sequencing with samples collected from six continents across distinct biogeographic conditions. We compared the gene catalog of citrus root microbiome with human and ocean microbiome gene catalogs. We also sequenced the amplicon of 16S V4 region and fungal ITS2 region for bulk soil, rhizosphere, and root samples across location. We defined the core citrus root-associated microbes and functions and determined the driving forces of the assembly process of plant root-associated microbiome at both microbial community and single genome (MAG) resolution. Our results broadened the genomic resource and view of plant root-associated microbiome, and shed lights on understanding the genomic and evolutionary bases of microbes for the host associated niche specification.</p> <p><strong>Key words: </strong>Citrus root-associated microbiome, metagenome, global, gene catalog</div>
|
|
