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Mechanism research of transcriptional regulator LeClp in the biosynthesis of WAP-8294A2 in Lysobacter enzymogenes

Huiyong Xu: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences

<div><em>Lysobacter enzymogene</em>s OH11, isolated from rhizosphere soil of pepper, is an important bacterial biocontrol agent. OH11 has a bright future in biocontrol application as it could produce WAP-8294A2. WAP-8294A2 is a crucial factor in biocontrol process of <em>L. enzymogenes</em> OH11. On the basis of sequencing genome, an important global regulator LeClp protein was identified from strain OH11 and shown to regulate the WAP-8294A2 biosynthesis via an indirect and uncharacterized mechanism(s). In the present study, we aim to identify the key factor(s) for modulating WAP-8294A2 biosynthesis in the downstream of LeClp by using ChIP-Seq、bacterial one-hybrid、EMSA、Co-IP、bacterial two-hybrid as well as other methods and elucidate the mechanism(s) of this factor(s) in the regulation of LeClp. Furthermore, it is another objective to understanding regulatory mechanism(s) of this factor(s) in modulating WAP-8294A2 biosynthesis. This results of the study is an important aid to generate genetically modified <em>L. enzymogens</em> strain with high-yeild WAP-8294A2.</div>