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Intensification on PAMP-triggered immunity confers disease resistance against bacterial soft rot

Zheng Jing-Lin: National Pingtung University of Science and Technology

<div>Bacterial soft rot disease is a devastating disease affecting a variety of plants worldwide. Possible strategy for controlling this disease consists of introducing the expression of the plant ferredoxin-like protein (pflp) gene in plants and usage of beneficial microorganisms. The PFLP-mediated resistance is associated with the intensification of PAMP-triggered immunity (PTI), have been demonstrated. To gain further insights regarding the enhanced intracellular PTI signaling contributed by PFLP,<em> Arabidopsis </em>mutants in MAPK pathway were used to assay the responses triggered by flg22<sub>Pst</sub>. Firstly, we confirmed that the rapid generation of H<sub>2</sub>O<sub>2</sub>, callose deposition, and hypersensitive response (HR) triggered by flg22<sub>Pst</sub> was intensified by PFLP. Then, we demonstrated that the flg22<sub>Pst</sub>-induced MAPK pathway was intensified by PFLP based on the expression of the <em>FRK1</em> gene mapk mutants. In addition to the rapid H<sub>2</sub>O<sub>2</sub> generation, callose deposition and expression of <em>FRK1 </em>were still intensified by <em>Bacillus amyloliquefaciens</em> PMB05 upon the treatment of flg22<sub>Pst</sub>. Morever, <em>B. amyloliquefaciens</em> PMB05 confers resistance against soft rot disease. These suggested that the disease resistance enhanced by <em>B. amyloliquefaciens</em> PMB05 was associated with the intensification on PTI through signaling in MAPK pathway. Taken together, we showed the intensification on PAMP-triggered immunity by PFLP or <em>B. amyloliquefaciens</em> strain PMB05 could be as a dominant factor to increase resistance against bacterial soft rot.</div>