Distribution and colonization of human opportunistic pathogen of Fusarium oxysporum in tomato
Chao-Jen Wang: National Chung Hsing University
<div><em>Fusarium oxysporum </em>(<em>Fo</em>) is widely distributed over soils, plant tissues, organic subtracts and water. <em>Fo</em> could cause Fusarium wilt in plants, and also make diseases on human such as keratitis, ringworm of nails and allergy etc. <em>Fo</em> is often found in the patient with low immunity. Thus, it is possible that the patients with low immunity have high risk to be infected by <em>Fo</em>. The Human Pathogens On Plants (HPOPs) are pay attention due to the risk of <em>Fo</em> on agricultural products. Moreover, the human opportunistic pathogen and plant pathogen of <em>Fo </em>were closely based on molecular phylogenetic analyses. Consequently, the human opportunistic pathogen of <em>Fo </em>might have chance to penetrate into the plant tissues. It is necessary to carry out whether the human opportunistic pathogen of <em>Fo </em>can penetrate into agricultural products. For detecting the human opportunistic pathogen of <em>Fo</em>, the specific primers (primer5/ST33-R) were developed based on the sequences polymorphism of intergenic spacer region (IGS) of ribosomal DNA from a human opportunistic pathogen of<em> Fo</em> isolates. In addition, the MCC2074 isolate was used to study the colonization and distribution in tomato plants and fruits. Results showed that the MCC2074 could colonize in tomato tissues by wound inoculation without symptoms showing. However, the MCC2074 was limited in roots, peg, hypocotyl and the basal stem tissues by using soil infestation method. The colonization of MCC2074 in tomato and cucumber fruits indicated that the MCC2074 could penetrate into fruits tissue and colonize in pericarp wall, locular cavity, and columella.</div>
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