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Critical role of cytochrome bc1 in tolerance of Xanthomonas campestris pv. campestris to Phenazine-1-carboxylic acid

Jian Wu: Nanjing agricultural university

<div><em>Xanthomonas</em> spp. is a class of plant Pathogen that infected a wide range plants, causing serious damage in global agriculture production. Phenazine-1 -carboxylic acid (PCA) is a microbial antibiotic, primarily produced by <em>Pseudomonas</em> spp. PCA had been used in control of rice bacterial leaf streak in rice planting region of South China. In previous study, we found twin-arginine translocation (Tat) system and cytochrome <em>c</em> synthesis (CCM) system were important in regulating the tolerance of PCA-insensitive bacterium <em>Xanthomonas campestris </em>pv.<em> campestris </em>(<em>Xcc</em>, the the causal agent of black rot of vegetable <em>brassica</em> crops). In our current study, we found that this phenomenon was caused by influencing the cytochrome bc1, which is the functional downstream of Tat system and CCM system. The EC<sub>50</sub> value of the cytochrome bc1 deletion mutant of <em>Xcc</em> to PCA decreased about 100 times than that of the WT. This results may provide some suggestions of the plant disease caused by <em>Xanthomonas</em> <em>campestris </em>pv.<em> campestris</em> in field control. In conclusion, the cytochrome bc1 was critical in tolerance of <em>Xanthomonas</em> <em>campestris </em>pv.<em> campestris</em> to PCA.</div>