Pyrosequencing-based microbial community analyses according to kiwi-biome organs
Min-Jung Kim: Department of plant medicine, Gyeongsang National University
<div><strong>Plants have a wide microbial diversity both on and inside of their tissues, which are rhizosphere, phyllosphere and endosphere. These microbes play important roles in healthiness of plant such as supply nutrition and enhance resistance against abiotic stresses or various pathogens. Understanding microbiome structure and diversity in plants are important information to reveal the microbial ecology and function in plant-microbe interaction. NGS techniques can lead to novel insights of phytobiome and reveal a relationship between environmental niches and evolution. This study assessed the bacterial and fungal diversity in kiwifruit rhizosphere, sap and pollen using the Illumina MiSeq platform. The experimental specimens were collected cv. Deliwoong kiwifruit tree (<em>Actinidia deliciosa</em>) in Namhae, South Korea. DNAs were extracted, 16S rRNA and ITS regions were amplified and produced amplicon libraries for MiSeq sequencing. In the rhizosphere, Firmicutes, Proteobacteria and Actinobacteria in bacteria and Ascomycota and Zygomycota in fungi occupied as major population. In the sap, Proteobacteria in bacteria and Ascomycota in fungi mostly occupied. Top 15 OTU fungi in the rhizosphere soils mostly belong to Zygomycota but in the saps mostly belong to Ascomycota. Top 15 OTU bacteria in the rhizosphere were belonged mostly phyla Actinobacteria and Firmicutes, genus <em>Streptomyces</em> spp. and <em>Bacillus </em>spp., which are known as PGPR strains and in saps were mostly phyla Proteobacteria and Actinobacteria, genus the others. These results showed that much taxa of PGPR and antagonistic microbes against pathogens can be targets for future research in tissue specific or shard microbes in kiwifruit.</strong></div>
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