Allelochemicals- A Significant Molecules for Control of Soil Borne Plant Pathogens from Rhizobacteria
Dr. Urja Pandya: Samarpan Science and Commerce College
<div>Rhizobacteria produced secondary compounds i.e. allelochemicals (like siderophores, antibiotics, volatile metabolites, enzymes, etc.) acts as signals against fungal pathogens. Present study described <em>in vitro</em> and <em>in vivo</em> biocontrol potential of selected strains against charcoal rot diseases of Groundnut (<em>Arachis hypogaea</em> (L.). The <em>Bacillus</em> strain MBCU2 (<em>Bacillus sonorensis</em>- KC741546) and MBCU4 (<em>Bacillus subtilis</em>- KC907389) showed maximum production of volatile metabolites, HCN production, lytic enzymes such as chitinase, β 1, 3 glucanase and cellulase production as compared to other strains. The scanning electron microscopy (SEM) analysis revealed loss of structural integrity of mycelia of <em>Macrophomina phaseolina </em>due to allelochemicals produced by biocontrol strains. <em>Bacillus</em> strain MBCU2 and MBCU4 recorded maximum groundnut growth enhancement with least disease incidence at 80<sup>th</sup> DAS (Day after sowing) over uninoculated control i.e. 21 and 28% respectively, under green house experiments. Also these biocontrol strains showed presence of active lipopeptides such as iturin, surfactin and fengycine, confirmed by LC-MS as well as MALDI-TOFF analysis. This study direct linked with integrated disease management strategies for crop protection.</div>
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