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Expression Analysis of Soybean PAL Induced by Biocontrol Rhizobia Sneb183

Yuanyuan Wang: Shenyang Agricultural University

<div>Abstract: As the most important enzyme in the metabolism of phenylpropanoids, PAL is involved in the synthesis and accumulation of plant secondary metabolites (phytoalexin, lignin and phenolic compounds), and plays an important role in plant disease resistance and can be used as a biochemical indicator of plant disease resistance. Biological and non-biological can induce plant PAL gene transcription at the transcriptional level. <em><i>Sinorhizobium fredii</i></em> Sneb183 was selected with ability to induce soybean resistance to SCN. To understand the mechanism of Sneb183 induce the soybean resistance,RtPCR and ELISA were used to investigated the expression patterns of PAL genes and the content of PAL in soybean root during the early stages different time points after Sneb183 inoculation. The results were as follows: RtPCR analysis results revealed that PAL mRNA levels reached to the highest value 4 hours after inoculation, 3.11 times that of the control, and decreased to 1.61 and 1.18 times from 6 to 8 hours after inoculation. It comes to1.81 times When 24 hours after inoculation. The PAL content in soybean roots has similar trend with the PAL mRNA. It is lower than control at 2 hours inoculated, but increases gradually after that. It has two peaks at 4 hours and 24hours after inoculation and 1.22 times and 1.14 times higher than the control respectively. Our results demonstrates that PAL in soybean roots can be induced by Sneb183, the different stage of PAL expression pattern has diverse role in infection, colonization of Sneb183 and resistance to SCN. At first PAL play adverse roles in Sneb183 infection and colonization, but after infection successfully, infected Sneb183 can rapidly induce the expression and accumulation of PAL in soybean root and resist SCN .</div>