A reliable glasshouse screening technique to detect BYDV-PAV disease resistance in cereal crops at early and late growth stage
Shormin Choudhury: TIA
<div>Barley yellow dwarf virus-PAV (BYDV-PAV) is one of the major virus causing a widespread and serious viral disease affecting cereal crops. A reliable method was developed to screen cereal crops (wheat, barley and oats) for BYDV-PAV resistance in glasshouse experiments. We evaluated traits associated with BYDV-PAV infection such as leaf discoloration, reduction in growth and yield traits, and percentage of virus-infected plants using ELISA and TBIA tests. Inoculation of plants using viruliferous aphids as vectors proved effective establishing infections and reducing plant growth in susceptible varieties, and in inducing leaf discoloration. Increasing the numbers of viruliferous aphids per plant led to very high percentages of infection in susceptible cultivars. Visual evaluation of symptoms on barley and oats is considered adequate for assessing BYDV-PAV resistance, but in wheat, it is necessary to evaluate BYDV-PAV resistance by an ELISA test (at early stage) and grain number and yield measurements (at late stage). Wheat varieties containing the <em>Bdv2 </em>gene demonstrated a high level of resistance for BYDV-PAV. The <em>Yd2</em> gene-containing barley variety showed leaf discoloration and plant height reduction but no significant reductions in yield, which suggests the need for yield analysis for assessing BYDV-PAV tolerance.</div>
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