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Botrytis cinerea control with Thymus vulgaris and Coriandrum sativum essential oils

Alma Valiuskaite: Lithuanian Research Centre for Agriculture and Forestry Institute of Horticulture

<div>The increasing demand for healthy and safe food and increasing resistance to chemical fungicides demanded new plant protection solutions. Nowadays consumers are concentrated on functional and natural food without pesticides residues. <em>Thymus vulgaris </em>and <em>Coriandrum sativum </em>essential oils were screened against <em>Botrytis cinerea</em>. Grey mould is one the of the main strawberry field and postharvest pathogen. The research was carried out at the LAMMC Institute of Horticulture. The <em>T. vulgaris </em>and <em>C. sativum</em> essential oils were extracted from local material. The inhibition of mycelial growth was evaluated at concentrations 200 µl/l, 400 µl/l, 600 µl/l, 800 µl/l and 1000 µl/l. Small purified isolate fragment of about 10-mm diameter was placed in the centre of a sterile Petri dish of PDA with different essential oil concentrations. Plates were incubated 22 <sup>o</sup> C in darkness and evaluated after 2, 4, 8, 10 days. The antifungal activity of <em>T. vulgaris </em>and <em>C. sativum</em> essential oils was compared with chemical fungicides (Signum and Switch 62,5 WG). The results showed that <em>T. vulgaris</em> essential oils inhibited completely of <em>B. cinerea </em>growth at concentrations > 400 μl/l. Mycelial growth of <em>B. cinerea</em> by <em>C. sativum</em> was inhibited only by 1000 µl/l. Therefore results of <em>T. vulgaris </em>essential oil study provide the basis for developing an effective biocontrol agent to control strawberry <em>B. cinerea</em>.</p> <p><strong>Keywords: </strong>Inhibition, Mycelial growth, Pathogens</div>