Whole Genome Sequencing and Secretome analysis of Tilletia indica inciting Karnal bunt of wheat Provides Pathogenesis-related genes
Malkhan Singh Gurjar: ICAR-Indian Agricultural Research Institute
<div><em>Tilletia indica</em> is an internationally quarantined plant pathogen inciting Karnal bunt of wheat. The disease was first reported from India in 1931. The pathogen is genetically highly diverse and unique life style which starts to infect the wheat plant at specific spike development stage. Therefore, <em>Tilletia</em> <em>indica</em> genome assembly size of 33.7 MB was generated with GC content of 55.0 % using HiSeq 2500 and PacBio RSII platforms. A total number of 10,113 genes were predicted with average gene size of 1,945 bp out of which functionally annotated genes were 7,262. A total number of 3,216 protein coding genes were assigned in different categories including biological process’ (1,148 genes), cellular (833 genes) and molecular function (1,235 genes). Out of a total number of 1,877 transposable elements, gypsy had the highest count. 5772 simple sequence repeat identified in the genome assembly. The most abundant simple sequence repeat type was trinucleotide having 42 % of all SSRs. In comparative genome analysis suggested 3,751 proteins of <em>Tilletia</em> <em>indica</em> had orthologs in five fungi whereas 126 proteins were unique. In comparative genome analysis suggested 3,751 proteins of <em>Tilletia</em> <em>indica</em> had orthologs in five fungi whereas 126 proteins were unique. Secretome analysis revealed the presence of 1,014 secretory proteins in the genome. Some putative pathogenicity-related genes were identified in genome. The full genome of <em>T</em>.<em> indica</em> will provide deeper understanding and provide window to understand the pathogenesis mechanism, fungal life cycle, survival of teliospores and novel strategies for management of Karnal bunt disease of wheat.</div>
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