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Occurrence of Grapevine fanleaf virus in Russia

Svetlana Vinogradova: Research Center of Biotechnology, Russian Academy of Sciences

<div><em>Grapevine fanleaf virus (GFLV)</em> is one of the most severe viruses of grapevine. It occurs worldwide, causes more than 50% yield loses and decrease sugar content and titratable acidity (Andret-Link et al., 2004). GFLV is transmitted by nematode <em>Xiphinema index. </em>To investigate the GFLV occurrence in Russia the survey of Crimea vineyards has been conducted in 2014-2017.</p> <p>Total of 937 leaf samples with virus symptoms were collected. Short internodes, zigzag growth of shoots, yellow discoloration of leaves were observed. Samples were analysed by RT-PCR with the specific to GFLV primers, followed by sequencing of the PCR products. GFLV was found in 2.3% of the samples. GFLV was detected in combination with RSPaV (0.51%). The combination of GFLV, GVA and GLRaV-1 occurs in 0.13% of samples and combination of GFLV, RSPaV and GLRaV-3 occurs in 0.13% of samples.</p> <p>Nowadays we develop immunochromatography assay for rapid GFLV detection in the vineyard.</p> <p>This work was performed using the experimental climate control facility U-73547, was funded by the Minobrnauka of Russia, Project № 14.607.21.0184, unique identifier FMEFI60717X0184.</div>