GC-MS metabolic pathways associated to the different stages of banana black sigatoka disease (BSD)
Maria Gabriela Mariduena-Zavala: Escuela Superior Politecnica del Litoral, ESPOL
<div>Banana (<em>Musa</em> <em>acuminata</em>) production is a significant source of income in many countries and black sigatoka (BSD) is one of the most devastating banana diseases worldwide. However, the metabolic changes occurring in bananas at the different stages of BSD are poorly understood. This work aimed to evaluate the metabolite profile of plants infected with BSD using a GC-MS. Four months old banana plants were inoculated with 1 x 10<sup>5</sup> cell/mL of <em>Pseudocercospora fijiensis—</em>the causal agent of BSD<em>—</em>ground mycelia and kept in a greenhouse. The disease progress was evaluated during six months and leaves with different symptomatic stages were sampled. Metabolites were extracted from symptomatic leaves using methanol, chloroform and water, followed by a derivatization using MSTFA (N-methyl-N- trimethylsilyl trifluoracetamide) and detection using GC-MS. Multivariate analysis revealed distinct metabolite profile of infected plants at different stages of BSD when compared with healthy plants. Various metabolites including Propanoic acid, Dotriocontano, L-Threonic acid, Ribonic acid, β-D-Galactopyranoside, Xylonic acid, Glucaric acid, Quercetin, Isochiapin B, Hexadecanoic acid, Nonadecanoic acid, D-(-)-Fructofuranose, D-(-)-Tagatofuranose, 4 D-(-)-Ribofuranose were significantly downregulated in infected plants while Xylonic acid, Octadecanoic acid, Glyceryl-glycoside, Glutamic acid, β-D-Allopyranose, Acetopyruvic acid, β-D-(-)-Ribopyranose, 2-Keto-I-gluconic acid, 3-α Mannobiose were significantly upregulated in the presence of BSD. Pathways associated to the disease progression include Flavone and flavonol, unsaturated fatty acids, Cutin, Phenylpropanoid, Flavonoid, Stilbenoid, diarylheptanoid and gingerol. Biosynthesis. This is the first report of metabolic changes occurring during BSD infection.</div>
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