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Novel Cytohabdovirus Identified in Native Rubus Exhibiting Virus-like Symptoms

Melinda Guzman: Oregon State University

<div>Raspberry leaf curl disease (RLCD) was first reported in the late 19th century in red and black raspberry and blackberry across northeastern United States and Canada. Symptoms of infection include small fruit, stunted shoots, leaf chlorosis, and downward curling of the leaves. <i>Aphis rubicola</i> transmits the disease in a persistent manner<i>.</i> Molecular tests for detection of this disease are not available. Combinations of <i>Black raspberry necrosis</i>, <i>Rubus yellow net</i>, <i>Raspberry leaf mottle</i>, a novel Enamovirus and two novel Cytorhabdoviruses were identified in a survey of native symptomatic <i>Rubus</i> from prevalent disease areas using next generation sequencing. Individual viruses were not consistently associated with symptoms observed in native <i>Rubus</i>. We focused on a Cytorhabdovirus detected in a <i>Rubus</i> from Pennsylvania because of its membership in one of two virus genera first associated with the disease. Primers were developed from the sequence of one of the detected Cytorhabdoviruses and used to test additional <i>Rubus </i>samples; the virus was detected in one symptomatic sample from Ontario, Canada. We determined the sequence of 14 kb of the genome of this Cytorhabdovirus. These molecular tools for the detection of the Cytorhabdovirus will be useful in studies to correlate its presence in planta and the development of RLCD.</div>