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Survey and Identification of Meloidogyne Species Associated with Potato in North Sumatra, Indonesia

Lisnawita Lisnawita: Universitas Sumatera Utara

<div>Root knot nematode (RKN<em>/</em><em>Meloidogyne</em> spp.) is one of the main constraint of potato production in Indonesia, especially in Karo District as potato production centre in North Sumatra. A survey in 9 localities of potato-growing area of Karo District was conducted to identify species of root-knot nematode associated with potato. Morphological characters based perrineal pattern, and DNA sequence analysis were used to identify the nematodes. Soil and root samples were collected from farmer’s fields. Single egg mass was identified by morphological studies and then confirmed by PCR amplification of DNA using species-specific SCAR (sequence characterized amplified region) primers, sequencing, and NCBI-BLASTn analysis of the target product. The results showed 3 species found in Karo District. They are <em>M. arenaria, M. incognita, </em>and <em>M. javanica. </em>The species were either found singly or in mixed populations. This is the first report of <em>Meloidogyne</em> species associated with potato in North Sumatra, Indonesia.</div>