Genome-wide analysis of NBS-LRR genes in Indian mustard (Brassica juncea) and prediction of candidate disease resistance genes
Fabian Inturrisi: University of Western Australia
<div><em></em><em>Brassica juncea</em> (Indian mustard) is a member of the economically important Brassicaceae family. A number of important diseases affect <em>B. juncea</em>, including blackleg and white rust. For crop improvement it’s important to identify resistance (R) genes for disease resistance breeding. In this study, a comprehensive genome–wide analysis of nucleotide binding site leucine-rich repeats (NBS-LRR) genes was performed in <em>B. juncea</em> along with prediction of candidate R genes from published resistance loci. The phylogenetic relationship between the <em>B. juncea</em> NBS-LRR genes was also studied. The 289 NBS-LRR genes identified in <em>B. juncea</em> were predominately TIR type and 45% were clustered. The physical position of published disease loci in <em>B. juncea</em> was identified through BLAST of markers on the reference genome. Four resistance loci were found for white rust resistance and seven loci were identified for blackleg resistance, with 28 NBS-LRR, 14 receptor-like proteins and 115 receptor-like kinases genes were located within and close by the regions. Eleven <em>B. juncea</em> public accessions were re-sequenced to determine genome-wide SNPs associated with the candidate R genes. Genome-wide analysis of <em>B. juncea</em> NBS-LRR genes provides a valuable resource to identify candidates for novel and functional R genes, which is a key step to enhance resistance against pathogens.</div>
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