Rapid detection and characterization of Phytophthora infestans isolations in the field.
Phillip Wharton: University of Idaho, Aberdeen Research and Extension Center
<div>Late blight of potato, caused by <em>Phytophthora infestans</em>, is a major constraint to potato production. In recent years, new genotypes, some with different epidemiological characteristics, have emerged in the global populations of <em>P. infestans</em>. Early detection, coupled with knowledge of the particular genotype present, can ensure the timely implementation of the most optimum disease management strategy. Recently, loop mediated isothermal amplification (LAMP), is becoming more widely used for the rapid on-site detection of plant pathogens. Similarly, allele specific oligonucleotide markers have been developed that can determine the genetic diversity of <em>P. infestans</em> using real time PCR. In this study, we developed a new LAMP assay for <em>P. infestans</em> which can easily distinguish <em>P. infestans</em> from other oomycetes such as <em>Phytophthora erythroseptica</em> within 20 minutes on a Genie IIIC platform. In addition, ASO-PCR was performed with US, European and Indonesian isolates. ASO-PCR profiles of the US reference isolates were concordant with those reported previously in Gagnon <em>et al</em>. (2016). ASO-PCR profiles also showed that the Indonesian isolates were different to the US isolates but more similar to the European Pink-6 isolates. LAMP and ASO-PCR, coupled with a portable platform such as the Genie IIIC, can therefore enable the rapid on-site detection and characterization of <em>P. infestans</em>.</div>
|
