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Population Genetics Analysis of Pythium myriotylum and Pythium aphanidermatum in Japan

Afandi Auliana: United Graduate School of Agricultural Science Gifu University

<div>The modern agriculture with hydroponic systems trigger the emerge of water borne pathogen. <em>Phythium myriotylum </em>and<em> P. aphanidermatum </em>are water borne pathogen that has wide variety of host plant and responsible for seedling rots, root rots, damping-off, root and stem-rot on many economically important crops. For better understanding of disease route and the genetic relatedness, the microsatellite markers for population genetics analyses for both pathogens were developed by genome sequence analysis. In total, six <em>P. myriotylum</em> primer sets with 29 alleles were used to analyze 82 isolates from 22 hosts plants and 20 prefectures. In the other hand, 11 <em>P. aphanidermatum</em> primer sets with 49 alleles were used to analyze 206 isolates from 13 host plants and 14 prefectures. The dendrogram made by UPGMA algorithm for <em>P. myriotlum</em> revealed that the isolates were rather clustered by their host plants than the geographical origin. On the other hand, the opposite result was obtained for <em>P. aphanidermatum</em>, it clustered by the geographical origin rather than the host plants. In conclusion, the different species might take different route to disperse.</div>

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