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Induction of defense by elicitors from different origins in Potato genotypes against Phytophthora infestans

Rafaela Lopes Martin: Agrocampus-Ouest / INRA UMR IGEPP

<div>In potato crop, <em>Phytophthora infestans</em>, the causal agent of late blight, is mainly controlled with pesticides. Biocontrol methods such as those inducing plant defenses could contribute to reduce their use. Several elicitors have been showed to induce potato defense responses in controlled conditions but they are not efficient in the field. Our hypothesis is that defense induction depends on both the origin of the applied elicitors and the potato genotype. To assess it two potato genotypes with different levels of resistance toward late blight were treated with PAMPs from <em>P.infestans, </em>a green algae extract or BABA. Four-week old leaf tissues were analysed 48 hours after treatment using non-target metabolomics analyses thanks to UPLC-QTOF-MS<sup>e</sup>. Ours results show that the accumulation of secondary metabolites such as phenylpropanoids, flavonoids and alkaloids are induced after elicitation, some of them (chlorogenic acid and rutin) being potential defense markers. Notably, chaconine, a specific molecule of potato, is more induced with the algae’s extract than with PAMPs. These metabolites will be validated and quantified with specific standards. Principal Components Analysis show the significantly difference between the genotypes. Moreover, to complete the investigation of our hypothesis, metabolomic datasets analysis is in progress to identify defense markers. Finally, in order to understand the defense induction pathways specific potato defense genes will be studied.</div>