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Role of hsp70 and calreticulin gene on temperature adapatation of Blumeria graminis f. sp. tritici

Jieru Fan: Institute of Plant Protection, CAAS

<div>Wheat powdery mildew, caused by <em>Blumeria graminis</em> f. sp.<em> tritici </em>(<em>Bgt</em>), is one of the most important wheat diseases worldwide. Temperature is considered to be an important climate factor affected disease epidemics and pathogen populations. As the climate warm up, over-wintering range of this disease is increased. Temperature tolerant strains have been isolated in the fields in China. To understand the basis of tolerance to temperature of <em>Bgt</em>, the expression level of heat shock protein 70 (<em>hsp70</em>) and calreticulin gene in temperature-tolerant and -sensitive isolates were investigated. The relative expression of <em>hsp70 </em>and calreticulin were increased in both phenotypes after heat-treat with 26ºC before inoculating on wheat seedlings compared with treatment at 18ºC, the optimum growth temperature for . In addition, the relative expression of <em>hsp70</em> and calreticulin is higher in temperature-tolerant strains in comparison with sensitive strains. These data suggested that hsp70 and calreticulin played important roles on adaptability to high temperature in <em>Bgt</em>.</div>