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Molecular Cytogenetic Characterization of a New T2BL·1RS Wheat-Rye Chromosome Translocation Line Resistant to Stripe Rust and Powdery Mildew

February 2009 , Volume 93 , Number  2
Pages  124 - 129

Chunmei Wang, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences (CAS), Shijiazhuang 050021, China; Qi Zheng, The State Key Laboratory of Plant Cell and Chromosome Engineering, IGDB, CAS, Beijing 100101, China; Lihui Li, The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China; Yongchun Niu, Institute of Plant Protection, CAAS, Beijing 100193, China, and Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China; Haibo Wang, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050051; Bin Li, The State Key Laboratory of Plant Cell and Chromosome Engineering, IGDB, CAS, Beijing 100101, China; and Xiaotian Zhang, Yunfeng Xu, and Diaoguo An, Center for Agricultural Resources Research, IGDB, CAS, Shijiazhuang 050021, China

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Accepted for publication 16 October 2008.

Wheat (Triticum aestivum) genotypes with rye (Secale cereale) 1RS chromosomal translocations are widely used in wheat breeding programs because 1RS carries genes for resistance to several diseases. However, some of the pathogens have evolved into new races that overcome the resistance due to extensive use of cultivars with the resistance genes from rye. Therefore, identification and deployment of new resistance sources with desirable agronomic characteristics are important and urgent. We have used winter rye cultivar German White as a source of genes for desirable traits in wheat improvement. A new genotype named WR04-32 was produced through hybridization and chromosome manipulation between common winter wheat cultivar Xiaoyan 6 and German White. This genotype was highly resistant to a wide spectrum of the wheat stripe rust (Puccinia striiformis f. sp. tritici) and powdery mildew (Blumeria graminis f. sp. tritici) pathotypes prevalent in China. The polymerase chain reaction (PCR) result using EST-STS (expressed sequence tag-site tagged sequence) marker STSWE126 specific to 1RS confirmed 1RS in WR04-32, and it was further proved to be a wheat-rye T2BL·1RS translocation line using sequential genomic in situ hybridization (GISH) and multicolor fluorescence in situ hybridization (FISH) with probes pAs1 and pSc119.2 (or pHvG38). In addition to its resistance to stripe rust and powdery mildew, WR04-32 was genetically stable and had desirable agronomic traits, making it a desirable germplasm for wheat breeding.

© 2009 The American Phytopathological Society